H941 Department für Angewandte Genetik und Zellbiologie (DAGZ)
Arbeitsgruppen
H94 Department für angewandte Genetik und Zellbiologie (DAGZ)
H941 Department für Angewandte Genetik und Zellbiologie (DAGZ) Arbeitsgruppen AG Steinkellner
Molekulare Pflanzenbiotechnologie
Gebiet
Expression rekombinanter Proteine in Pflanzen, molekulare Grundlagen |
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Ausrichtung
Die Arbeitsgruppe beschäftigt sich mit Grundlagenforschung mit anwendungsorientierter Ausrichtung. Ziel ist die optimale Nutzung von Pflanzen zur rekominanten Erzeugung von therapeutisch wertvollen Glykoproteinen. Die Forschung umfasst die Etablierung einer innovativen Technologieplattform die es ermöglicht neue Konzepte zur „persönlichen“ Therapie aufgrund eines speziellen Glykosylierungsmusters der Zielproteine umzusetzen. Das beinhaltet Arbeiten an der molekularen Charakterisierung des planzlichen N-Glycosylierungs- Biosyntheseweges, N-glycan Engineering und rekombinante Expression von Zielproteinen.
Projekte
laufende Projekte
abgeschlossene Projekte
Laura Bassi Center of Expertise
Ausgewählte Publikationen (ab 2005)
Zeitlin L, Pettitt J, Scully C, Bohorova N, Kim D, Pauly M, Hiatt A, Ngo L, Steinkellner H, Whaley KJ, Olinger GG. (2011) Enhanced potency of a fucose-free monoclonal antibody being developed as an Ebola virus immunoprotectant. Proc Natl Acad Sci U S A. 108:20690-4.
Castilho A, Bohorova N, Grass J, Bohorov O, Zeitlin L, Whaley K, Altmann F, Steinkellner H. (2011) Rapid high yield production of different glycoforms of Ebola virus monoclonal antibody. PLoS One. 2011;6(10):e26040
Loos A, Van Droogenbroeck B, Hillmer S, Grass J, Pabst M, Castilho A, Kunert R, Liang M, Arcalis E, Robinson DG, Depicker A, Steinkellner H (2011) Expression of antibody fragments with a controlled N-glycosylation pattern and induction of endoplasmic reticulum-derived vesicles in seeds of Arabidopsis. Plant Physiol. 155:2036-48.
Castilho A, Gattinger P, Grass J, Jez J, Pabst M, Altmann F, Gorfer M, Strasser R, Steinkellner H. (2011). N-glycosylation engineering of plants for the biosynthesis of glycoproteins with bisected and branched complex N-glycans. Glycobiology. 21:813-23.
Loos A, Van Droogenbroeck B, Hillmer S, Grass J, Kunert R, Cao J, Robinson DG, Depicker A, Steinkellner H. (2011) Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana. Plant Biotechnol J. 9:179-92.
Forthal DN, Gach JS, Landucci G, Jez J, Strasser R, Kunert R, Steinkellner H. (2010) Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12. J Immunol.185:6876-82.
Sequential Depletion and Acquisition of Proteins during Golgi Stack Disassembly and Reformation. Schoberer J, Runions J, Steinkellner H, Strasser R, Hawes C, Osterrieder A.
(2010) Traffic 11:1429-44.
Castilho A, Strasser R, Stadlmann J, Grass J, Jez J, Gattinger P, Kunert R, Quendler H, Pabst M, Leonard R, Altmann F, Steinkellner H. (2010) In planta protein sialylation through overexpression of the respective mammalian pathway. J Biol Chem. 285:15923-30.
F1000 Biology (must read) http://www.f1000biology.com/article/id/3063956
Strasser, R., Castilho, A., Stadlmann, J., Kunert, R., Quendler, H., Gattinger, P., Jez, J., Rademacher, T., Altmann, F., Mach, L., and Steinkellner, H. (2009)
Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile. J. Biol. Chem. 284, 20479-85.
Schoberer, J., Vavra, U., Stadlmann, J., Hawes, C., Mach, L., Steinkellner, H., Strasser R. (2009). Arginine/lysine residues in the cytoplasmic tail promote ER export of plant glycosylation enzymes. Traffic 10, 101-15.
Castilho, A., Pabst, M., Altmann, F., Mach, L., Glössl, J., Strasser, R., and Steinkellner, H. (2008) Engineering CMP-sialic acid (CMP-Neu5Ac) biosynthesis into plants. Plant Physiology : 147, 331-9.
Strasser, R., Stadlmann, J., Schähs, M., Stiegler, G., Quendler, H., Mach, L. Glössl, J., and Steinkellner, H. (2008) Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure. Plant Biotechnol. J. 6, 392-402.
Strasser, R., Bondili, J.S., Vavra, U., Schoberer, J., Svoboda, B., Glössl, J., Leonard, R., Stadlmann, J., Altmann, F., Steinkellner, H., and Mach, L. (2007). A unique beta1,3-galactosyltransferase is indispensable for the biosynthesis of N-Glycans containing Lewis a structures in Arabidopsis thaliana. Plant Cell 19, 2278-92.
Jin, C., Altmann, F., Strasser, R., Mach, L., Schähs, M., Kunert, R., Rademacher, T., Glössl, J., and Steinkellner H. (2008) A plant derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits. Glycobiology 18, 235-41.
Schähs, M., Strasser, R., Stadlmann, J., Kunert, R., Rademacher, T., and Steinkellner, H. (2007). Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern. Plant Biotechnol. J. 5, 657-663.
Strasser, R., Bondili, J.S., Schoberer, J., Svoboda, B., Liebminger, E., Glössl, J., Altmann, F., Steinkellner,H., and Mach,L. (2007). Enzymatic properties and subcellular localization of Arabidopsis beta-N-acetylhexosaminidases. Plant Physiol. 7, 2278-92.
Strasser, R., Schoberer, J., Jin, C., Glössl, J., Mach, L., and Steinkellner, H. (2006). Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants. Plant J. 45, 789-803.
Bencur, P., Steinkellner, H., Svoboda, B., Mucha, J., Strasser, R., Kolarich, D., Hann, S., Kollensperger, G., Glössl, J., Altmann, F., and Mach,L. (2005). Arabidopsis thaliana beta1,2-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathway. Biochem. J. 388, 515-525.
Strasser, R., Altmann, F., Mach, L., Glössl, J., and Steinkellner, H. (2004). Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta 1,2-linked xylose and core alpha 1,3-linked fucose. FEBS Letters 561, 132-136.
Forschungsförderungsorganisationenen
| Fonds zur Förderung der Wissenschaftlichen Forschung (Austrian Science Funds) |
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Kooperationen
- Department für Chemie, Universität für Bodenkultur Wien; contactperson: Dr. Friedrich Altmann
Department of Biotechnology, BOKU-WIEN, contactperson: Renate Kunert, Karola Vorauer-Uhl
Medical University Vienna, Institute for Pathophysiology,
contactperson: Eva Untersmayr-ElsenhuberRheinisch-Westfälische Technische Hochschule, Aachen, D: contactperson: Thomas Rademacher
Oxford Brooks University, Oxford UK , contactperson: Chris Hawes
Universität Heidelberg, D, contactperson: David G. Robinson
University Gent, B, contactperson: Ann Depicker
UC Irvine School of Medicine, USA, contactperson: Donald Forthal
Arizona State University, USA contactperson: Charles Arntzen
Scripps Research Institute, La Jolla, USA, contactperson: Heribert Quendler
Bayer BioScience, NV, Gent, B, contactperson: Koen Weterings
Mapp biopharmaceuticals, Inc, San Diego, USA, contactperson: Kevin Whaley
f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H., Wien, Austria,
contactperson: Max Woisetschläger