Zellbiologie, Zellwand der Pflanze, Arabidopsis Genetik
Leiter: Dipl.-Ing. Dr. Georg Seifert
Plant cell walls represent the world’s most abundant natural resource for fuel, fibre, food and fodder. Despite the cell wall’s importance for human society much remains to be learned about its biosynthesis and its central role in plant life. Cell walls consist of cellulose microfibrils that are embedded in an amorphous matrix of cross-linking glucans, acidic pectins and highly glycosylated proteins such as extensins and arabinogalactan-proteins. The work of this group is dedicated to better understand the biosynthesis and functions of cell wall matrix polymers. One of our areas of interest is the regulation of cell wall carbohydrate precursor metabolism by multiple isoforms of nucleotide sugar interconversion enzymes, potentially leading to approaches to modulate cell wall matrix composition and thereby improving processing properties. Another long-term interest of our group is the investigation of plant cell wall performance and integrity control. This process that is best understood in yeast, is predicted to control plant growth in response to abiotic stress and pathogen attack. Its elucidation promises strategies to make crop biomass production less sensitive to abiotic stress.
Figure 1: Polarized light micrography of hypocotyl sections of a wild type plant (left) and the mur10 mutant (right) that lacks secondary cell wall specific cellulose synthase. Strongly birefringent normal secondary cell wall produces polarization contrast while mutant secondary cell walls only contain ligin (dark blue).
Figure 2: Immunosilver labelled cell wall matrix polymers in secondary thickened hypocotyls in Arabidopsis thaliana wild type and mutant plants defective in UDP-galactose biosynthesis.
Figure 3: Mutations in the arabinogalactan-protein FLA4/SOS5 causes oversensitivity to salt, resulting in abnormally swollen and highly autofluorescent roots (left). Secondary mutations in several suppressor loci of fla4 (SLOF) restore wild type responsiveness to salt (right). SLOF genes are predicted to act in cell wall performance and integrity control. Seifert et al. (2010) Cell Wall Meeting 2010 Porto, Portugal
Luschnig C. Seifert G.(2010): Post-translational modifications of plasma membrane proteins and their implications for plant growth and development. In: A. Murphy, B. Schulz and W. Peer Eds., Plant Plasma Membrane; Plant Cell Monographs , 109-128; Springer-Verlag , Heidelberg, Germany; ISBN 978-3-642-13430-2
Seifert, GJ; Blaukopf, C(2010): Irritable Walls: The Plant Extracellular Matrix and Signaling. PLANT PHYSIOL. 2010; 153(2): 467-478.[Fulltext] [PubMed] [Fulltext BOKU-login] [Listed in ISI Web of Science]
Wieczorek, K., El Ashry, A., Quentin, M., Seifert, G., Favery, B., Grundler, F.M.W., Bohlmann, H.(2010): Pectin and pectin degradation in nematode feeding structures. In: ESN/BOKU Wien, 30th International Symposium of the European Society of Nematologists - Proceedings [Poster]
[30th International Symposium of the European Society of Nematologists, Vienna, Austria, SEP 19-23, 2010]
Wieczorek, K., El Ashry, A., Quentin, M., Seifert, G., Favery, B., Grundler, F.M.W., Bohlmann, H.(2010): Pectin and pectin degradation in nematode feeding structures . In: COST 872, NEMAGENICS - Exploiting genomics to understand plant-nematode interactions Proceedings of the FOURTH Annual Meeting [Poster]
[COST 872, European Cooperation in the field of Science and Technology, NEMAGENICS - Exploiting genomics to undertand plant-nematode interactions - Proceedings of the FOURTH Annual Meeting, Lisbon, PORTUGAL, Mai 24-27, 2010]
Wieczorek, K., Seifert, G. and Grundler, F.M.W.(2010): Cell wall remodelling and biosynthesis during pathogenesis of cyst nematodes . In: University of Porto, The XII Cell Wall Meeting - abstract book [Poster]
[The XII Cell Wall Meeting, Porto, PORTUGAL, JULY 25-30, 2010]