Microbial Genetics - Fungal Genomics Unit
Fungal transcription factors and epigenetics
We try to understand at the molecular level how fungal transcription factors work in the context of chromatin to find their targets and to function according to the “instructions” they receive through signaling pathways from their environment. With these questions in mind we work on the saprophytic species Aspergillus nidulans and on two plant-pathogenic Fusarium species which infect wheat, maize and rice.
We use molecular genetic tools for functional studies of genes in our fungi and do lots of biochemistry work to isolate and to characterize the transcription factor proteins. Genome-wide studies of protein localization (ChIP-seq) and transcriptional activity (RNA-seq) are routinely used techniques in our lab. We also perform plant infection studies and use ChIP and RNA-seq to understand how these regulatory factors influence virulence of the pathogens. Finally, in cooperation with the AIT group (Markus Gorfer), we are interested to learn how such regulatory networks impose on the fitness and ecological functions of fungi in their environments.
Leader: Univ.-Prof. Mag. Dr. Joseph Strauss
Current scientific projects and cooperations
The biosynthesis pathway and function of nitric oxide (NO*) – a short lived radical – on transcriptional networks in the nitrogen circuit and on developmental pathways in Aspergillus nidulans.
Austrian Science Fund (FWF) project together with David Canovas (Lise Meitner Project 2015-2017) in which we already determined that the nitrate reductase is one of the biosynthesis routes, but there are other pathways under examination which can contribute to NO* production in Aspergillus nidulans.
Epigenetic regulation of fungal pathogens: the role of histone regulators in the interactions between Fusarium species and crops plants
We have worked since many years on the chromatin-level regulation of primary metabolism, e.g. we showed that AreA, a broad-spectrum nitrogen regulator, is required for chromatin remodeling (Muro-Pastor et al., 1999, 2004) and that AreA brings histone acetylation to target promoters (Berger et al.,2006, 2008 ). Similarly we found a connection between heterochromatin and secondary metabolite gene expression (Bok et al., 2009, Reyes-Dominguez et al., 2010). In the frame of a large Research Network funded by the FWF (Special Research Area SFB-Fusarium, 2016-2019) we can study selected histone modifiers in Fusarium and Aspergillus species for their role in regulation of metabolism and virulence.
Finding novel virulence factors by an epigenetic approach
A Lise-Meitner project (2017-2019) of the FWF to Lena Studt will dig deeper into the question how epigenetic factors influence virulence. Lena is using the rice pathogen Fusarium fujikuroi as a model system to ask which genes with known or presumed histone modification activities are vital for the infection process of this notorius pathogen. Results from these studies will help us to better understand the relevance of the epignetic dimension in host-pathogen interactions.