News & Events


New FWF-Project


IgG Subclass Glycosylation

Project lead: Herta Steinkellner

I 3721 Internationale Projekte
Start: 01.06.2018

New publication from Kleine-Vehn Lab

The Road to Auxin-Dependent Growth Repression and Promotion in Apical Hooks

The phytohormone auxin controls growth rates within plant tissues, but the underlying mechanisms are still largely enigmatic. The apical hook is a superb model to understand differential growth, because it displays both auxin-dependent growth repression and promotion. In this special issue on membranes, we illustrate how the distinct utilization of vesicle trafficking contributes to the spatial control of polar auxin transport, thereby pinpointing the site of growth repression in apical hooks  » https://doi.org/10.1016/j.cub.2018.01.069

New publication from Kleine-Vehn Lab

PIN7 Auxin Carrier Has a Preferential Role in Terminating Radial Root Expansion in Arabidopsis thaliana

Abstract
Directional growth of lateral roots is critical for radial expansion and soil coverage. Despite its importance, almost nothing is known about its molecular determinants. Initially, young lateral roots (LRs) grow away from the parental root, maintaining the angle acquired shortly after emergence. A second downwards bending response to gravity terminates the so-called plateau phase and thereby limits radial root expansion  » doi:10.3390/ijms19041238

New publication from Hauser Lab

Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2α phosphorylation

Abstract
One of the main mechanisms blocking translation after stress situations is mediated by phosphorylation of the α-subunit of the eukaryotic initiation factor 2 (eIF2), performed in Arabidopsis by the protein kinase GCN2 which interacts and is activated by ILITHYIA(ILA). ILA is involved in plant immunity and its mutant lines present phenotypes not shared by the gcn2 mutants » doi.org/10.1016/j.jplph.2018.04.003

Faszination Pflanze: Warum die Karotte orange ist

Jürgen Kleine-Vehn im Blog der Junge Akademie der ÖAW über die Faszination Pflanze.

Hier geht es zum »Blog  
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Twitter:
• Jürgen Kleine-Vehn  @KleineVehnLab 
• Junge Akademie der ÖAW  @ya_OeAW

New book on Root Development edited by Elke Barbez (Kleine-Vehn Lab)

Introduction
This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment  » Methods in Molecular Biology, vol 1761

New publication from Korbei Lab and Kleine-Vehn Lab

Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

Abstract
Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions  » doi.org/10.1007/978-1-4939-7747-5_16

New publication from Kleine-Vehn Lab

Growth Rate Normalization Method to Assess Gravitropic Root Growth

Abstract
Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature  » doi.org/10.1007/978-1-4939-7747-5_15

New publication from Kleine-Vehn Lab

Cortical Cell Length Analysis During Gravitropic Root Growth

Abstract
The typical parameter used to evaluate the root growth response to gravity is the degree of root bending in time. This employs the quantification of the root tip angle toward gravity and, hence, does not directly assess the actual differential growth process. Here, we describe the cortical cell length as a parameter to quantify cell elongation during the gravitropic response, using median longitudinal confocal sections  » doi.org/10.1007/978-1-4939-7747-5_14

Congratulations Alexandra!


New FWF Stand-Alone Project: 

N. benthamiana ß-galactosidases acting on glycoproteins

Project lead: Alexandra Castilho

New publication from Adam Lab:

UDP-Glucosyltransferases from Rice, Brachypodium, and Barley: Substrate Specificities and Synthesis of Type A and B Trichothecene-3-O-β-D-glucosides

Abstract
Trichothecene toxins are confirmed or suspected virulence factors of various plant-pathogenic Fusarium species. Plants can detoxify these to a variable extent by glucosylation, a reaction catalyzed by UDP-glucosyltransferases (UGTs). Due to the unavailability of analytical standards for many trichothecene-glucoconjugates, information on such compounds is limited  » full text

PhD Defense of Somanath Kallolimath

Production of recombinant proteins with polysialylated N-glycans in Nicotiana benthamiana

23.02.2018 - 14:00
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Herta Steinkellner

PhD Defense of Chloé Béziat

Characterization of PILS putative intracellular auxin carrier family during developmental processes in Arabidopsis

22.02.2018 - 14:30
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Jürgen Kleine-Vehn

New Publication from Steinkellner/Strasser Lab:

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinantglycoproteins produced in Nicotiana benthamiana.

Summary
N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central protein-complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticulum (ER)  » doi.org/10.1111/pbi.12906

New publication from Adam Lab:

Chemical synthesis of culmorin metabolites and their biologic role in culmorin and acetyl-culmorin treated wheat cells

The Fusarium metabolite culmorin (1) is receiving increased attention as an “emerging mycotoxin”. It cooccurs with trichothecene mycotoxins and potentially influences their toxicity. Its ecological role and fate in plants is unknown. We synthesized sulfated and glucosylated culmorin conjugates as potential metabolites, which are expected to be formed in planta, and used them as reference compounds. An efficient procedure for the synthesis of culmorin sulfates was developed  » DOI: 10.1039/c7ob02460f

New Publication from Strasser Lab:

The glycan-dependent ERAD machinery degrades topologically diverse misfolded proteins.

Summary
A great number of soluble and integral membrane proteins fold in the endoplasmic reticulum (ER) with the help of chaperones and folding factors. Despite these efforts, protein folding is intrinsically error prone and amino acid changes, alterations in posttranslational modifications or cellular stress can cause protein misfolding. Folding-defective non-native proteins are cleared from the ER and typically undergo ER-associated degradation (ERAD)  » doi.org/10.1111/tpj.13851

New publication from Hauser Lab:

Root hair abundance impacts cadmium accumulation in Arabidopsis thaliana shoots

Background and Aims
Root hairs increase the contact area of roots with soil and thereby enhance the capacity for solute uptake. The strict hair/non-hair pattern of Arabidopsis thaliana can change with nutrient deficiency or exposure to toxic elements, which modify root hair density. The effects of root hair density on cadmium (Cd) accumulation in shoots of arabidopsis genotypes with altered root hair development and patterning were studied  » doi.org/10.1093/aob/mcx220

Latest pre-print from Elena Feraru (Kleine-Vehn Lab):

PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

Abstract
Global warming is threatening plant productivity, because plant growth is highly sensitive to elevated temperatures. High temperature (HT) triggers the auxin biosynthesis-dependent growth in aerial tissues. On the other hand, the contribution of auxin to HT-induced root growth is currently under debate. Here we show that the putative intracellular auxin carrier PIN-LIKES 6 (PILS6) is a negative regulator of organ growth and that its abundance is highly sensitive to HT  » get the pre-print