Paper from BiMM and Schüller Lab with AKH

"Antifungal susceptibility of yeast blood stream isolates collected during a 10 year period in Austria"

Candida‐associated infections put a significant burden on western health care systems. Development of (multi‐) resistant fungi can become untreatable and threaten especially vulnerable target groups, such as the immunocompromised.

We assessed antifungal susceptibility and explored possible influence factors of clinical Candida isolates collected from Austrian hospitals between 2007 and 2016

» https://doi.org/10.1111/myc.12892

» BiMM Bioactive Microbial Metabolites

» Schüller Lab

Publication with contribution from Hauser Lab

"Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments"


  • UV-B exposure increased expression of flavonoid and UVR8 genes
  • Accumulation of most phenolics occurred 36 h after UV-B irradiation
  • 60 min-UVB-exposure determined a greater phenolic accumulation than 10 min-UVB
  • Flavonoids, particularly anthocyanins, were the most UV-B-affected phenolics
  • UV-B treatment determined a higher complexity level of anthocyanins after 36 h

» https://doi.org/10.1016/j.plaphy.2018.11.009

» Hauser Lab

Publication with contribution from Adam Lab

"The role of roughage provision on the absorption and disposition of the mycotoxin deoxynivalenol and its acetylated derivatives in calves: from field observations to toxicokinetics."

A clinical case in Belgium demonstrated that feeding a feed concentrate containing considerable levels of deoxynivalenol (DON, 1.13 mg/kg feed) induced severe liver failure in 2- to 3-month-old beef calves. Symptoms disappeared by replacing the highly contaminated corn and by stimulating ruminal development via roughage administration. A multi-mycotoxin contamination was demonstrated in feed samples collected at 15 different veal farms in Belgium...

» https://doi.org/10.1007/s00204-018-2368-8

» Adam Lab

Publication with contribution from Adam Lab

"Untargeted LC–MS based 13C labelling provides a full mass balance of deoxynivalenol and its degradation products formed during baking of crackers, biscuits and bread"


  • Deoxynivalenol (DON) is mostly stable during the baking process.
  • isoDON, norDON B and norDON C were identified as DON degradation products.
  • The major thermal degradation product of DON is isoDON.
  • isoDON is far less toxic to ribosomes than DON.

» https://doi.org/10.1016/j.foodchem.2018.11.150

» Adam Lab

Publication from Adam Lab

"Cross-reactivity of commercial and non-commercial deoxynivalenol-antibodies to emerging trichothecenes and common deoxynivalenol-derivatives"

Immunoassay based techniques are an important and fast option for the detection and quantification of mycotoxins. They are frequently used as on-site screening tools in grain elevators, storage and production facilities. However, accurate quantification may be hampered by the co-recognition of structurally related metabolites by the used antibodies. Therefore, it is crucial to assess their cross-reactivity to avoid misinterpretation of the results...

» https://www.wageningenacademic.com/doi/pdf/10.3920/WMJ2018.2363

» Adam Lab

International PhD Programme “Biomolecular Technology of Proteins (BioToP)”

at the Vienna Institute of BioTechnology of BOKU
University of Natural Resources and Life Sciences, Vienna, Austria

» fully-funded PhD student positions for three years

BioToP offers an inter- and multi-disciplinary research-based doctoral education at the interface of basic and applied science in the field of protein biotechnology.
BioToP offers a challenging scientific environment with state-of-the-art facilities and provides comprehensive and thorough up-to-date research training in the fields of:

  • structure-function analysis, engineering and design of proteins
  • protein synthesis, targeting and post-translational modifications
  • expression systems and cell factories
  • bioinformatics and molecular modelling

The BioToP-specific educational programme comprises lectures, seminars and instructional courses that complement the research work in the participating groups. Highly qualified and motivated students of any nationality are invited to apply for the 3-year studentships. Funding will be according to the salary scheme of the Austrian Science Fund.
Additionally students will receive funding for research stays abroad and for the participation at international conferences.

Further information on research projects as well as application guidelines and forms are available at:  http://biotop.boku.ac.at

Application deadline: February 13th, 2019

Publication from Lena Studt (Strauss Lab)

"The putative H3K36 demethylase BcKDM1 affects virulence, stress responses and photomorphogenesis in Botrytis cinerea"


  • T-DNA insertion in the virulence-attenuated mutant PA2810 disrupts bckdm1.
  • Bckdm1 encodes a putative histone 3 lysine 36-specific demethylase. 
  • Bckdm1 is required for virulence, stress responses and photomorphogenesis.
  • Orthologs from other Ascomycetes cannot replace BcKDM1.

» https://doi.org/10.1016/j.fgb.2018.11.003

» Strauss Lab

Impromptu Seminar of Dr. Surinder Chopra

"Maize and Sorghum Flavonoid Pigments: From Genetic and Epigenetic Markers to Plant Health Applications"
(Penn State University, Department of Plant Sciences)

The goal of Dr. Surinder Chopra’s research is to develop a better understanding of metabolic coordination and role of secondary metabolites in plant developmental process as well as plant resistance to biotic and abiotic stresses. His group uses a flavonoid biosynthetic pathway in maize and sorghum as a model system. The research has led to the identification of a genetic factor that is involved in the biosynthesis of a class of plant flavonoid compounds, which act as anti-fungal agents in sorghum. These antifungal metabolites are also known as phytoalexins and in sorghum these compounds belong to the 3-deoxyanthocyanidin category. The group is now generating sorghum and maize mutants and introgression lines/over expression breeding lines to understand the regulation of defense compounds. In addition to the biotic stress, they are screening and developing new sorghum accessions that are tolerant to colder climatic regions.

19. December 2018, 11:00-11:45
Seminarraum Angewandte Genetik (MUG2-04/54)
Muthgasse 18, 1190 Wien

Guest Professor at the DAGZ

David Cánovas research interests are focused on the signals that control fungal development and reproduction using Aspergillus nidulans and Neurospora crassa as model organisms.

941001 VU Genetics of fungal development (in Eng.)  BOKU online
06.12.2018 - 14.12.2018, UFT Tulln, 3 ECTS

  • 06.12.2018: 09:00 14:00 (Lab E2.4.170)
  • 07.12.2018: 09:00 14:00 (Lab E2.4.170)
  • 10.12.2018: 09:00 15:00 (Lab E2.4.170)
  • 11.12.2018: 09:00 15:00 (Lab E2.4.170)
  • 12.12.2018: 09:00 14:00 (Lab E2.4.170)
  • 13.12.2018: 09:00 14:00 (Lab E2.4.170)
  • 14.12.2018: 09:00 14:00 (Lab E2.4.170)

David Cánovas studied Pharmacology at the University of Sevilla and received his Ph.D. degree in Pharmacology with a focus on Molecular and Cellular Biology in 1999. He received further training as a postdoc, first in environmental microbiology and then in fungal genetics, in the National Center for Biotechnology – CSIC (Madrid, Spain) and the University of Melbourne (Australia). He was appointed as a tenure track (within the competitive national “Ramon y Cajal” program) in the Department of Genetics – University of Sevilla in 2008. He was appointed Associate Professor in 2012. Currently he is associate editor of several international journals. 

He was recently the recipient of a Lise Meitner project from the FWF to study the role of nitric oxide in fungal reproduction at the DAGZ, which finished in 2017 and has already resulted in several publications.

Preprint from Kalyna Lab

"Remote control of alternative splicing in roots through TOR kinase"

For plants, light is the source of energy and the most relevant regulator of growth and adaptations to the environment by inducing changes in gene expression at various levels, including alternative splicing. Chloroplasts trigger retrograde signals that control alternative splicing in leaves and roots in response to light. 


Kalyna Lab

Publication From Strauss Lab

"Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites"

The eukaryotic epigenetic machinery can be modified by bacteria to reprogram the response of eukaryotes during their interaction with microorganisms. We discovered that the bacterium Streptomyces rapamycinicus triggered increased chromatin acetylation and thus activation of the silent secondary metabolism ors gene cluster in the fungus Aspergillus nidulans


Strauss Lab

DAGZ Seminar 11.12.2018

Alexander Jones
Sainsbury Laboratory

"The makings of a gradient: visualising plant hormones in vivo""

Tuesday 11.12.2018
09:00 am
DAGZ seminar room (4th floor, Muthgasse 18, 1190 Wien) 
Host: Sascha Waidmann

Kleine-Vehn Lab

BiRT Lecture Series

Dr. Jan-Peter George
BFW Bundesforschungszentrum für Wald
Institut für Waldgenetik

"Molecular genetics reveal putative adaptive variation in resistance against Chrysomyxa rhododendri in Norway spruce"

Thursday November 22nd, 2018
Time: 10:00
Campus Tulln / UFT – Seminar Room 14
Host: Joseph Strauss & Markus Gorfer

Strauss Lab

VIBT Seminar

Kris Vissenberg
IMPRES (Integrated Molecular Plant physiology Research)
Biology Department, University of Antwerp, Belgium

"Control of root hair development in Arabidopsis thaliana"

Friday 09.11.2018, 13:00
DAGZ seminar room (MUG2-04/54)
Host: Marie-Theres Hauser

Hauser Lab

BiRT Seminar Series „die Welt der biologischen Interaktionen“

Friedrich Kragler Univ. Doz. Dr.
Max Planck Institute of Molecular Plant Physiology, Wissenschaftspark Golm Germany

"Lost in translation: messenger RNA moving to distant tissues"

Wann: 14. November 2018, 10:00
Wo: Campus Tulln / UFT – Seminarraum 17'
Host: Christoph Schüller

Schüller Lab

Die BiMM und das Internet der Pilze

Auf der Suche nach Links im Internet der Pilze 
Können Schimmelpilze Informationen über die Abwehr von Bakterien an das gesamte Pilznetzwerk weitergeben und speichern?
Die Antwort auf diese Frage könnte bei der Entwicklung neuer Antibiotika und Krebstherapien helfen » zum Artikel

Strauss Lab

BiMM Bioactive Microbial Metabolites

Publication from Kalyna Lab

"PRP4KA, a Putative Spliceosomal Protein Kinase, Is Important for Alternative Splicing and Development in Arabidopsis thaliana"

Splicing of pre-mRNAs is an essential step in the expression of most eukaryotic genes. Both constitutive splicing and alternative splicing, which produces multiple mRNA isoforms from a single primary transcript, are modulated by reversible protein phosphorylation. Although the plant splicing machinery is known to be a target for phosphorylation, the protein kinases involved remain to be fully defined...

The article has been chosen as a highlight for the December issue of GENETICS. http://www.genetics.org/content/210/4/NP


Kalyna Lab

Publication from Schüller Lab

"A constitutive active allele of the transcription factor Msn2 mimicking low PKA activity dictates metabolic remodeling in yeast"

In yeast, protein kinase A (PKA) adjusts transcriptional profiles, metabolic rates and cell growth in concordance with carbon source availability. PKA affects gene expression mostly via the transcription factors Msn2 and Msn4, two key regulators of the environmental stress response. Here we analyzed the role of the PKA-Msn2 signaling module by using an Msn2 allele that harbors serine to alanine substitutions at six functionally important PKA motifs (Msn2A6)...


Schüller Lab

FWF Einzelprojekt für Richard Strasser

"Der Oligosaccharyltransferase-Komplex von Pflanzen"

Projektnummer: P 31920 Einzelprojekte

ProjektleiterIn: Richard STRASSER

Kuratoriumssitzung: 01.10.2018

Strasser Lab

“Endoplasmic reticulum-associated degradation of glycoproteins”

Richard Strasser talks about “Endoplasmic reticulum-associated degradation of glycoproteins” at the GMI seminar.

Friday, 19 October 2018, 11:30am @ GMI, Orange Seminar Room.

Gregor Mendel Institute of Molecular Plant Biology GmbH
Dr. Bohr-Gasse 3, 1030 Vienna

Strasser Lab

BiRT Seminar Series „die Welt der biologischen Interaktionen“

Prof. Ivo Frébort
Palacký University Olomouc, Czech Republic

"Plants as a tool for sustainable global development - Genetic improvement of barley and molecular farming"

Wann: 18. Oktober 2018, 9:30
Wo: Campus Tulln / UFT – Seminarraum 14

Publication from Strauss Lab

Fungus found floating in the Danube river - now identified and described by BiMM team as new fungal species: 
"Metapochonia lutea, a new species isolated from the Danube river in Austria"

A new species Metapochonia lutea (Ascomycota, Hypocreales) is described and illustrated. This fungus differs from the other taxa in the genus Metapochonia by its production of intensive yellow pigment in culture, conidiophores with relatively complex verticillate branching, bean-shaped conidia, and by delayed development of one-celled and prominent thick-walled submerged chlamydospores usually in chains or irregular clusters of 3–5 cells... 


Strauss Lab

Publication from Adam Lab

"Impact of glutathione modulation on the toxicity of the Fusarium mycotoxins deoxynivalenol (DON), NX-3 and butenolide in human liver cells."

DON, NX-3 and butenolide (BUT) are secondary metabolites formed by Fusarium graminearum. Evidence for formation of DON-glutathione adducts exists in plants, and also in human liver (HepG2) cells mass spectrometric evidence for GSH-adduct formation was reported. NX-3 is a DON derivative lacking structural features for Thiol-Michael addition, while BUT has the structural requirements (conjugated double bond and keto group)...


Adam Lab

Publication from Adam Lab

"New Plasmids for Fusarium Transformation Allowing Positive-Negative Selection and Efficient Cre-loxP Mediated Marker Recycling"

In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created a series of transformation vectors that allow antibiotic-based selection of transformants and subsequent negative selection for marker removal using thymidine kinase fusions combined with the Cre-loxP system... 


Adam Lab

Hands-on Exhibition for children "Earth & Soil"

Our department is a cooperation partner for an upcoming exhibition of the Zoom museum.
This hands on exhibition on "Earth and Soil" is for children ages 6-12.
Running time: 27. September 2018 - 24. February 2019

Zoom Kindermuseum

Kleine-Vehn Lab

Congratulations Verena!

Congratulation to Verena Unterwurzacher to finalzing her PhD Thesis with an excellent defense!
Thesis was done over the last years in collaboration with the AIT group of Markus Gorfer.
Topic: "Development of a multiplex qPCR detection systems for fungal indoor contamination"

Strauss Lab


At the 10th ÖGMBT meeting Somanath Kallolimath was awarded with the ÖGMBT Life Science PhD Award 2018 (sponsored by THP medical products).

Steinkellner Lab

WKO Preis 2018 für Andreas Schüller (Strauss Lab)

Andreas Schüller stellt sein Projekt vor, welches mit dem Wirtschaftskammerpreis 2018 an der BOKU ausgezeichnet wurde. Es geht um die Etablierung eines neuen Systems zur gezielten Aktivierung und Entdeckung von bioaktiven Stoffen in filamentösen Pilzen.


Strauss Lab

"Development of the Next Generation Influenza Vaccine"

Seminar of Marc-André D’Aoust Vice-President, Research and Innovation Medicago, inc, CAN

Thurs, 4th Oct 2018, 2 pm
Seminar room Department of Applied Genetics and Cell Biology (Muthgasse 18, 4th floor)

Host: Herta Steinkellner, Richard Strasser, Alexandra Castilho


The BOKU (Jürgen Kleine-Vehn), GMI (Yasin Dagdas) and the University of Vienna (Verena Ibl) have organized (4th-7th September 2018) together the 21st Meeting of the European Network of Plant Endomembrane Research (ENPER)  #ENPER 2018
150 plant cell biologists attended the meeting.

» Kleine-Vehn Lab
» Dagdas Lab
» Ibl Lab

FWF Project for Lena Studt 

"Role of the histone variant H2A.Z in phytopathogenic fusaria"

project number:  I 3911 Internationale Projekte

decision board: 25.06.2018

» Lena Studt

Publication from Seifert Lab

"A speculation on the tandem fasciclin 1 repeat of FLA4 proteins in angiosperms"

The Arabidopsis thaliana Fasciclin like arabinogalactan protein 4 (FLA4) locus is required for normal root growth in a linear genetic pathway with the FEI1 and FEI2 loci coding for receptor-like kinases. The two Fas1 domains of FLA4 are onserved among angiosperms but only the C-terminal Fas1 domain is required for genetic function...

» https://doi.org/10.1080/15592324.2018.1507403

» Seifert Lab

»Just cut it - an easy way to turn a negative regulator to positive mode-of-action« Publication from Strauss Lab

"Truncation of the transcriptional repressor protein Cre1 in Trichoderma reesei Rut-C30 turns it into an activator"

The filamentous fungus Trichoderma reesei is a natural producer of cellulolytic and xylanolytic enzymes and is therefore industrially used. Many industries require high amounts of enzymes, in particular cellulases. Strain improvement strategies by random mutagenesis yielded the industrial ancestor strain Rut-C30... 

» https://doi.org/10.1186/s40694-018-0059-0

» Strauss Lab

Publication from Schüller Lab

"Competition of Candida glabrata against Lactobacillus is Hog1 dependent"

Candida glabrata is a common human fungal commensal and opportunistic pathogen. This fungus shows remarkable resilience as it can form recalcitrant biofilms on indwelling catheters, has intrinsic resistance against azole antifungals and is causing vulvo‐vaginal candidiasis. As a nosocomial pathogen, it can cause life‐threatening bloodstream infections in immune‐compromised patients... 

» https://doi.org/10.1111/cmi.12943

» Schüller Lab

Publication from Hauser Lab

"Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions"

Resolving functions of closely linked genes is challenging or nearly impossible with classical genetic tools. Four members of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family are clustered on Arabidopsis chromosome five. To resolve the potentially redundant functions of this subclass of CrRLK1Ls named MEDOS1 to 4 (MDS1 to 4), we generated a single CRISPR/Cas9 transformation vector using a Golden Gate based cloning system to target all four genes simultaneously... 

» https://doi.org/10.1038/s41598-018-30711-3

» Hauser Lab

Publication from Lena Studt (Strauss Lab)

"Set1 and Kdm5 are antagonists for H3K4 methylation and regulators of the major conidiation‐specific transcription factor gene ABA1 in Fusarium fujikuroi"

Here we present the identification and characterisation of the H3K4‐specific histone methyltransferase Set1 and its counterpart, the Jumonji C demethylase Kdm5, in the rice pathogen Fusarium fujikuroi. While Set1 is responsible for all detectable H3K4me2/me3 in this fungus, Kdm5 antagonises the H3K4me3 mark. Notably, deletion of both SET1 and KDM5 mainly resulted in the upregulation of genome‐wide transcription, also affecting a large set of secondary metabolite (SM) key genes... 

» https://doi.org/10.1111/1462-2920.14339

» Strauss Lab

Preprint from Korbei Lab

"TOL proteins mediate vacuolar sorting of the borate transporter BOR1 in Arabidopsis thaliana"

Boron (B) is an essential micronutrient for plants, however, it shows cytotoxicity at high concentrations. A borate transporter BOR1 is required for efficient transport of boron (B) toward the root stele in Arabidopsis thaliana. BOR1 shows polar localization in the plasma membrane of various root cells toward the stele-side under B limitation. 

» https://doi.org/10.1101/342345

» Korbei Lab

Josef Glössl zum Urteil des Europäischen Gerichtshofs über den Einsatz neuer Mutagenese Methoden:

"Ich bin von diesem Urteil sehr enttäuscht, weil ich es in den wesentlichen Punkten nicht für sachlich begründet und nachvollziehbar halte"

zum Artikel  

zum Urteil im Wortlaut

Gesichter der BOKU - David Cánovas

Interview mit David Cánovas Mitarbeiter Fungal Genomics Unit - Universitäts- und Forschungszentrum Tulln


» Strauss Lab

Publication from Adam Lab

"Response of intestinal HT-29 cells to the trichothecene mycotoxin deoxynivalenol and its sulfated conjugates"

The sulfated forms of the Fusarium toxin deoxynivalenol (DON), deoxynivalenol-3-sulfate (DON-3-Sulf) and deoxynivalenol-15-sulfate (DON-15-Sulf) were recently described, however little is known about their mechanism of action in mammalian cells... 

» https://doi.org/10.1016/j.toxlet.2018.07.007

» Adam Lab

PhD Defense of Julia Richter  - passed with distinction!

"The role of cell wall integrity receptors in responses to heavy metals"

Zeit: Mittwoch, 11.07.2018, 10:00
Ort: Seminarraum Angewandte Genetik (MUG2-04/54)
Muthgasse 18, 4. Stock

Supervisor: Marie-Theres Hauser

Publication from Mach Lab

"The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities"

The genome of the model plant Arabidopsis thaliana encodes three paralogues of the papain-like cysteine proteinase cathepsin B (AtCathB1, AtCathB2 and AtCathB3), whose individual functions are still largely unknown.

» https://doi.org/10.1515/hsz-2018-0186

» Mach Lab

FWF Elise-Richter Programm for Elena Feraru

Auxin homeostasis in plant organ responses to high temperature

project number: V 690 Richter-Programm

decision board: 25.06.2018

» Elena Feraru

FWF Elise-Richter Programm for Doris Lucyshyn

O-GlcNAc and O-fucose binding plant lectins

project number: V 658 Richter-Programm

decision board: 25.06.2018

» Lucyshyn Lab

FWF Stand-Alone Project for Christian Luschnig

Ubiquitin E3 Ligases and Cell Polarity in Arabidopsis

project number: P 31493 Einzelprojekte

decision board: 25.06.2018

» Luschnig Lab

Agrargenetik und Biodiversität - Exkursion 20.06.2018

Das war auch heuer wieder ein spannender Exkursionstag im Zoo Schönbrunn mit Vorträgen und Backstage-Führungen für unsere Studierenden des Bachelorstudiums Agrarwissenschaften.
Die Themen: Warum ist Biodiversität nur mit genetischer Diversität denkbar, warum ist Artenschutz essentiell und was sind die Gründe für die „Kernschmelze der Agrar-Biodiversität“ in den letzten Jahrzehnten? 

Diese und viele weitere Fragen wurden von den Studierenden mit den Veranstaltern diskutiert. Ein Angebot des DAGZ im Zuge der Lehrveranstaltung "941091 Agrargenetik und Biodiversität - Exkursion". 

Publication from Steinkellner Lab

"Advanced Plant-Based Glycan Engineering"

With respect to biomanufacturing, glycosylation is one of the most addressed post-translational modifications, since it is well-known that the attachment of sugar residues efficiently affects protein homogeneity and functionality. Much effort has been taken into engineering various expression systems to control glycosylation and to generate molecules with targeted sugar profiles. 

» https://doi.org/10.3389/fbioe.2018.00081

» Steinkellner Lab

Vortrag zum Thema "Der EU-Sortenschutz, gewerblicher Rechtsschutz für Pflanzensorten"

Der dazu eingeladene Experte, Dirk Theobal kommt vom Gemeinschaftlichen Sortenamt (CPVO) und wird über die Funktionsweise des gemeinschaftlichen Systems zum Schutz von Pflanzensorten, die technischen Schutzanforderungen an Sorten und die rechtlichen Verfahren berichten (die Agenda finden Sie im Anhang)

Donnerstag, 14.06.2018, von 09:00 bis 12:00 Uhr  

Department für Angewandte Genetik und Zellbiologie (DAGZ): Muthgasse 18, (MUG2-04/54)

Publication from Seifert Lab

"Fascinating Fasciclins: A Surprisingly Widespread Family of Proteins that Mediate Interactions between the Cell Exterior and the Cell Surface"

The Fas1 proteins act as mediators between the cell and its environment and function in similar fashion in processes as diverse as human brain cancer and pollen formation in plants.This review for the first time compares the biology of Fas1 proteins from all kingdoms of life.

» https://doi.org/10.3390/ijms19061628

» Seifert Lab

Josef Glössl zum stellvertretenden Vorsitzenden des Universitätsrats der Medizinischen Universität Innsbruck gewählt  

Professor Josef Glössl, gerade eben aus dem Rektorat der BOKU (Vizerektor für Forschung und Internationale Beziehungen) an das DAGZ zurückgekehrt, wurde vor Kurzem zum stellvertretenden Vorsitzenden des Universitätsrats der Medizinischen Universität Innsbruck gewählt. 

Herzliche Gratulation!

» Universitätsrat der Medizinischen Universität Innsbruck 

Visit from the Wake Forest University

Elizabeth Sarkel, a junior biochemistry and molecular biology major from Columbus, Ohio is joining the Kleine-Vehn Lab again this summer. Elizabeth received a Fulbright Program fellowship and will work on gravitropic set point angle of lateral roots.


• The Fulbright Program @FulbrightPrgrm
• Wake Forest University @WakeForest
• Kleine-Vehn Lab  @KleineVehnLab 

FWF-Project for Herta Steinkellner

IgG Subclass Glycosylation

Project lead: Herta Steinkellner

I 3721 Internationale Projekte
Start: 01.06.2018

Publication from Seifert Lab

"Mad moves of the building blocks – nucleotide sugars find unexpected paths into cell walls" 

This short review tracks the unexpectedly yet purposefully complex, intracellular journey of an activated sugar before it safely reaches its destination in plant fibre polysaccharides.

» https://doi.org/10.1093/jxb/ery026

» Seifert Lab

Publication from Kleine-Vehn Lab

"The Road to Auxin-Dependent Growth Repression and Promotion in Apical Hooks"

The phytohormone auxin controls growth rates within plant tissues, but the underlying mechanisms are still largely enigmatic. The apical hook is a superb model to understand differential growth, because it displays both auxin-dependent growth repression and promotion. In this special issue on membranes, we illustrate how the distinct utilization of vesicle trafficking contributes to the spatial control of polar auxin transport, thereby pinpointing the site of growth repression in apical hooks.

» https://doi.org/10.1016/j.cub.2018.01.069

» Kleine-Vehn Lab

Publication from Kleine-Vehn Lab

"PIN7 Auxin Carrier Has a Preferential Role in Terminating Radial Root Expansion in Arabidopsis thaliana"

Directional growth of lateral roots is critical for radial expansion and soil coverage. Despite its importance, almost nothing is known about its molecular determinants. Initially, young lateral roots (LRs) grow away from the parental root, maintaining the angle acquired shortly after emergence. A second downwards bending response to gravity terminates the so-called plateau phase and thereby limits radial root expansion 

» doi:10.3390/ijms19041238

» Kleine-Vehn Lab

Publication from Hauser Lab

"Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2α phosphorylation" 

One of the main mechanisms blocking translation after stress situations is mediated by phosphorylation of the α-subunit of the eukaryotic initiation factor 2 (eIF2), performed in Arabidopsis by the protein kinase GCN2 which interacts and is activated by ILITHYIA(ILA). ILA is involved in plant immunity and its mutant lines present phenotypes not shared by the gcn2 mutants

» doi.org/10.1016/j.jplph.2018.04.003

» Hauser Lab

Faszination Pflanze: Warum die Karotte orange ist

Jürgen Kleine-Vehn im Blog der Junge Akademie der ÖAW über die Faszination Pflanze.

Hier geht es zum »Blog  

• Jürgen Kleine-Vehn  @KleineVehnLab 
• Junge Akademie der ÖAW  @ya_OeAW

Book on Root Development edited by Elke Barbez (Kleine-Vehn Lab)

This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment  » Methods in Molecular Biology, vol 1761

Publication from Korbei Lab and Kleine-Vehn Lab

Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions  » doi.org/10.1007/978-1-4939-7747-5_16

Publication from Kleine-Vehn Lab

Growth Rate Normalization Method to Assess Gravitropic Root Growth

Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature  » doi.org/10.1007/978-1-4939-7747-5_15

Publication from Kleine-Vehn Lab

Cortical Cell Length Analysis During Gravitropic Root Growth

The typical parameter used to evaluate the root growth response to gravity is the degree of root bending in time. This employs the quantification of the root tip angle toward gravity and, hence, does not directly assess the actual differential growth process. Here, we describe the cortical cell length as a parameter to quantify cell elongation during the gravitropic response, using median longitudinal confocal sections  » doi.org/10.1007/978-1-4939-7747-5_14

Congratulations Alexandra!

New FWF Stand-Alone Project: 

N. benthamiana ß-galactosidases acting on glycoproteins

Project lead: Alexandra Castilho

Publication from Adam Lab

UDP-Glucosyltransferases from Rice, Brachypodium, and Barley: Substrate Specificities and Synthesis of Type A and B Trichothecene-3-O-β-D-glucosides

Trichothecene toxins are confirmed or suspected virulence factors of various plant-pathogenic Fusarium species. Plants can detoxify these to a variable extent by glucosylation, a reaction catalyzed by UDP-glucosyltransferases (UGTs). Due to the unavailability of analytical standards for many trichothecene-glucoconjugates, information on such compounds is limited  » full text

PhD Defense of Somanath Kallolimath

Production of recombinant proteins with polysialylated N-glycans in Nicotiana benthamiana

23.02.2018 - 14:00
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Herta Steinkellner

PhD Defense of Chloé Béziat

Characterization of PILS putative intracellular auxin carrier family during developmental processes in Arabidopsis

22.02.2018 - 14:30
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Jürgen Kleine-Vehn

Publication from Steinkellner/Strasser Lab

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinantglycoproteins produced in Nicotiana benthamiana.

N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central protein-complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticulum (ER)  » doi.org/10.1111/pbi.12906

Publication from Adam Lab:

Chemical synthesis of culmorin metabolites and their biologic role in culmorin and acetyl-culmorin treated wheat cells

The Fusarium metabolite culmorin (1) is receiving increased attention as an “emerging mycotoxin”. It cooccurs with trichothecene mycotoxins and potentially influences their toxicity. Its ecological role and fate in plants is unknown. We synthesized sulfated and glucosylated culmorin conjugates as potential metabolites, which are expected to be formed in planta, and used them as reference compounds. An efficient procedure for the synthesis of culmorin sulfates was developed  » DOI: 10.1039/c7ob02460f

Publication from Strasser Lab

The glycan-dependent ERAD machinery degrades topologically diverse misfolded proteins.

A great number of soluble and integral membrane proteins fold in the endoplasmic reticulum (ER) with the help of chaperones and folding factors. Despite these efforts, protein folding is intrinsically error prone and amino acid changes, alterations in posttranslational modifications or cellular stress can cause protein misfolding. Folding-defective non-native proteins are cleared from the ER and typically undergo ER-associated degradation (ERAD)  » doi.org/10.1111/tpj.13851

Publication from Hauser Lab:

Root hair abundance impacts cadmium accumulation in Arabidopsis thaliana shoots

Background and Aims
Root hairs increase the contact area of roots with soil and thereby enhance the capacity for solute uptake. The strict hair/non-hair pattern of Arabidopsis thaliana can change with nutrient deficiency or exposure to toxic elements, which modify root hair density. The effects of root hair density on cadmium (Cd) accumulation in shoots of arabidopsis genotypes with altered root hair development and patterning were studied  » doi.org/10.1093/aob/mcx220

Pre-print from Elena Feraru (Kleine-Vehn Lab):

PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

Global warming is threatening plant productivity, because plant growth is highly sensitive to elevated temperatures. High temperature (HT) triggers the auxin biosynthesis-dependent growth in aerial tissues. On the other hand, the contribution of auxin to HT-induced root growth is currently under debate. Here we show that the putative intracellular auxin carrier PIN-LIKES 6 (PILS6) is a negative regulator of organ growth and that its abundance is highly sensitive to HT  » get the pre-print