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Publication from Adam Lab

"Response of intestinal HT-29 cells to the trichothecene mycotoxin deoxynivalenol and its sulfated conjugates"

The sulfated forms of the Fusarium toxin deoxynivalenol (DON), deoxynivalenol-3-sulfate (DON-3-Sulf) and deoxynivalenol-15-sulfate (DON-15-Sulf) were recently described, however little is known about their mechanism of action in mammalian cells... 

» https://doi.org/10.1016/j.toxlet.2018.07.007

» Adam Lab

PhD Defense of Julia Richter  - passed with distinction!

"The role of cell wall integrity receptors in responses to heavy metals"

Zeit: Mittwoch, 11.07.2018, 10:00
Ort: Seminarraum Angewandte Genetik (MUG2-04/54)
Muthgasse 18, 4. Stock

Supervisor: Marie-Theres Hauser

Publication from Mach Lab

"The two cathepsin B-like proteases of Arabidopsis thaliana are closely related enzymes with discrete endopeptidase and carboxydipeptidase activities"

The genome of the model plant Arabidopsis thaliana encodes three paralogues of the papain-like cysteine proteinase cathepsin B (AtCathB1, AtCathB2 and AtCathB3), whose individual functions are still largely unknown.

» https://doi.org/10.1515/hsz-2018-0186

» Mach Lab

FWF Elise-Richter Programm for Elena Feraru

Auxin homeostasis in plant organ responses to high temperature

project number: V 690 Richter-Programm

decision board: 25.06.2018

» Elena Feraru

FWF Elise-Richter Programm for Doris Lucyshyn

O-GlcNAc and O-fucose binding plant lectins

project number: V 658 Richter-Programm

decision board: 25.06.2018

» Lucyshyn Lab

FWF Stand-Alone Project for Christian Luschnig

Ubiquitin E3 Ligases and Cell Polarity in Arabidopsis

project number: P 31493 Einzelprojekte

decision board: 25.06.2018

» Luschnig Lab

Agrargenetik und Biodiversität - Exkursion 20.06.2018

Das war auch heuer wieder ein spannender Exkursionstag im Zoo Schönbrunn mit Vorträgen und Backstage-Führungen für unsere Studierenden des Bachelorstudiums Agrarwissenschaften.
Die Themen: Warum ist Biodiversität nur mit genetischer Diversität denkbar, warum ist Artenschutz essentiell und was sind die Gründe für die „Kernschmelze der Agrar-Biodiversität“ in den letzten Jahrzehnten? 

Diese und viele weitere Fragen wurden von den Studierenden mit den Veranstaltern diskutiert. Ein Angebot des DAGZ im Zuge der Lehrveranstaltung "941091 Agrargenetik und Biodiversität - Exkursion". 

Publication from Steinkellner Lab

"Advanced Plant-Based Glycan Engineering"

With respect to biomanufacturing, glycosylation is one of the most addressed post-translational modifications, since it is well-known that the attachment of sugar residues efficiently affects protein homogeneity and functionality. Much effort has been taken into engineering various expression systems to control glycosylation and to generate molecules with targeted sugar profiles. 

» https://doi.org/10.3389/fbioe.2018.00081

» Steinkellner Lab

Vortrag zum Thema "Der EU-Sortenschutz, gewerblicher Rechtsschutz für Pflanzensorten"

Der dazu eingeladene Experte, Dirk Theobal kommt vom Gemeinschaftlichen Sortenamt (CPVO) und wird über die Funktionsweise des gemeinschaftlichen Systems zum Schutz von Pflanzensorten, die technischen Schutzanforderungen an Sorten und die rechtlichen Verfahren berichten (die Agenda finden Sie im Anhang)

Donnerstag, 14.06.2018, von 09:00 bis 12:00 Uhr  

Department für Angewandte Genetik und Zellbiologie (DAGZ): Muthgasse 18, (MUG2-04/54)

Publication from Seifert Lab

"Fascinating Fasciclins: A Surprisingly Widespread Family of Proteins that Mediate Interactions between the Cell Exterior and the Cell Surface"

The Fas1 proteins act as mediators between the cell and its environment and function in similar fashion in processes as diverse as human brain cancer and pollen formation in plants.This review for the first time compares the biology of Fas1 proteins from all kingdoms of life.

» https://doi.org/10.3390/ijms19061628

» Seifert Lab

Josef Glössl zum stellvertretenden Vorsitzenden des Universitätsrats der Medizinischen Universität Innsbruck gewählt  

Professor Josef Glössl, gerade eben aus dem Rektorat der BOKU (Vizerektor für Forschung und Internationale Beziehungen) an das DAGZ zurückgekehrt, wurde vor Kurzem zum stellvertretenden Vorsitzenden des Universitätsrats der Medizinischen Universität Innsbruck gewählt. 

Herzliche Gratulation!

» Universitätsrat der Medizinischen Universität Innsbruck 

Visit from the Wake Forest University

Elizabeth Sarkel, a junior biochemistry and molecular biology major from Columbus, Ohio is joining the Kleine-Vehn Lab again this summer. Elizabeth received a Fulbright Program fellowship and will work on gravitropic set point angle of lateral roots.
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Twitter:

• The Fulbright Program @FulbrightPrgrm
• Wake Forest University @WakeForest
• Kleine-Vehn Lab  @KleineVehnLab 

FWF-Project for Herta Steinkellner


IgG Subclass Glycosylation

Project lead: Herta Steinkellner

I 3721 Internationale Projekte
Start: 01.06.2018

Publication from Seifert Lab

"Mad moves of the building blocks – nucleotide sugars find unexpected paths into cell walls" 

This short review tracks the unexpectedly yet purposefully complex, intracellular journey of an activated sugar before it safely reaches its destination in plant fibre polysaccharides.

» https://doi.org/10.1093/jxb/ery026

» Seifert Lab

Publication from Kleine-Vehn Lab

"The Road to Auxin-Dependent Growth Repression and Promotion in Apical Hooks"

The phytohormone auxin controls growth rates within plant tissues, but the underlying mechanisms are still largely enigmatic. The apical hook is a superb model to understand differential growth, because it displays both auxin-dependent growth repression and promotion. In this special issue on membranes, we illustrate how the distinct utilization of vesicle trafficking contributes to the spatial control of polar auxin transport, thereby pinpointing the site of growth repression in apical hooks.

» https://doi.org/10.1016/j.cub.2018.01.069

» Kleine-Vehn Lab

Publication from Kleine-Vehn Lab

"PIN7 Auxin Carrier Has a Preferential Role in Terminating Radial Root Expansion in Arabidopsis thaliana"

Directional growth of lateral roots is critical for radial expansion and soil coverage. Despite its importance, almost nothing is known about its molecular determinants. Initially, young lateral roots (LRs) grow away from the parental root, maintaining the angle acquired shortly after emergence. A second downwards bending response to gravity terminates the so-called plateau phase and thereby limits radial root expansion 

» doi:10.3390/ijms19041238

» Kleine-Vehn Lab

Publication from Hauser Lab

"Arabidopsis ILITHYIA protein is necessary for proper chloroplast biogenesis and root development independent of eIF2α phosphorylation" 

One of the main mechanisms blocking translation after stress situations is mediated by phosphorylation of the α-subunit of the eukaryotic initiation factor 2 (eIF2), performed in Arabidopsis by the protein kinase GCN2 which interacts and is activated by ILITHYIA(ILA). ILA is involved in plant immunity and its mutant lines present phenotypes not shared by the gcn2 mutants

» doi.org/10.1016/j.jplph.2018.04.003

» Hauser Lab

Faszination Pflanze: Warum die Karotte orange ist

Jürgen Kleine-Vehn im Blog der Junge Akademie der ÖAW über die Faszination Pflanze.

Hier geht es zum »Blog  
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Twitter:
• Jürgen Kleine-Vehn  @KleineVehnLab 
• Junge Akademie der ÖAW  @ya_OeAW

Book on Root Development edited by Elke Barbez (Kleine-Vehn Lab)

Introduction
This detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment  » Methods in Molecular Biology, vol 1761

Publication from Korbei Lab and Kleine-Vehn Lab

Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue

Abstract
Here, we present different methods for immunoprecipitating membrane proteins of Arabidopsis thaliana root material. We describe two extraction methods for the precipitation either for an integral membrane protein of the endoplasmic reticulum (ER) or a peripheral membrane protein partially localized at the plasma membrane, where we precipitate the protein out of the total membrane as well as total cytosolic fractions  » doi.org/10.1007/978-1-4939-7747-5_16

Publication from Kleine-Vehn Lab

Growth Rate Normalization Method to Assess Gravitropic Root Growth

Abstract
Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature  » doi.org/10.1007/978-1-4939-7747-5_15

Publication from Kleine-Vehn Lab

Cortical Cell Length Analysis During Gravitropic Root Growth

Abstract
The typical parameter used to evaluate the root growth response to gravity is the degree of root bending in time. This employs the quantification of the root tip angle toward gravity and, hence, does not directly assess the actual differential growth process. Here, we describe the cortical cell length as a parameter to quantify cell elongation during the gravitropic response, using median longitudinal confocal sections  » doi.org/10.1007/978-1-4939-7747-5_14

Congratulations Alexandra!


New FWF Stand-Alone Project: 

N. benthamiana ß-galactosidases acting on glycoproteins

Project lead: Alexandra Castilho

Publication from Adam Lab

UDP-Glucosyltransferases from Rice, Brachypodium, and Barley: Substrate Specificities and Synthesis of Type A and B Trichothecene-3-O-β-D-glucosides

Abstract
Trichothecene toxins are confirmed or suspected virulence factors of various plant-pathogenic Fusarium species. Plants can detoxify these to a variable extent by glucosylation, a reaction catalyzed by UDP-glucosyltransferases (UGTs). Due to the unavailability of analytical standards for many trichothecene-glucoconjugates, information on such compounds is limited  » full text

PhD Defense of Somanath Kallolimath

Production of recombinant proteins with polysialylated N-glycans in Nicotiana benthamiana

23.02.2018 - 14:00
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Herta Steinkellner

PhD Defense of Chloé Béziat

Characterization of PILS putative intracellular auxin carrier family during developmental processes in Arabidopsis

22.02.2018 - 14:30
DAGZ-Seminarroom (MUG2-04/54)
Muthgasse 18, 4th floor

Supervisor: Jürgen Kleine-Vehn

Publication from Steinkellner/Strasser Lab

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinantglycoproteins produced in Nicotiana benthamiana.

Summary
N-glycosylation is critical for recombinant glycoprotein production as it influences the heterogeneity of products and affects their biological function. In most eukaryotes, the oligosaccharyltransferase is the central protein-complex facilitating the N-glycosylation of proteins in the lumen of the endoplasmic reticulum (ER)  » doi.org/10.1111/pbi.12906

Publication from Adam Lab:

Chemical synthesis of culmorin metabolites and their biologic role in culmorin and acetyl-culmorin treated wheat cells

The Fusarium metabolite culmorin (1) is receiving increased attention as an “emerging mycotoxin”. It cooccurs with trichothecene mycotoxins and potentially influences their toxicity. Its ecological role and fate in plants is unknown. We synthesized sulfated and glucosylated culmorin conjugates as potential metabolites, which are expected to be formed in planta, and used them as reference compounds. An efficient procedure for the synthesis of culmorin sulfates was developed  » DOI: 10.1039/c7ob02460f

Publication from Strasser Lab

The glycan-dependent ERAD machinery degrades topologically diverse misfolded proteins.

Summary
A great number of soluble and integral membrane proteins fold in the endoplasmic reticulum (ER) with the help of chaperones and folding factors. Despite these efforts, protein folding is intrinsically error prone and amino acid changes, alterations in posttranslational modifications or cellular stress can cause protein misfolding. Folding-defective non-native proteins are cleared from the ER and typically undergo ER-associated degradation (ERAD)  » doi.org/10.1111/tpj.13851

Publication from Hauser Lab:

Root hair abundance impacts cadmium accumulation in Arabidopsis thaliana shoots

Background and Aims
Root hairs increase the contact area of roots with soil and thereby enhance the capacity for solute uptake. The strict hair/non-hair pattern of Arabidopsis thaliana can change with nutrient deficiency or exposure to toxic elements, which modify root hair density. The effects of root hair density on cadmium (Cd) accumulation in shoots of arabidopsis genotypes with altered root hair development and patterning were studied  » doi.org/10.1093/aob/mcx220

Pre-print from Elena Feraru (Kleine-Vehn Lab):

PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

Abstract
Global warming is threatening plant productivity, because plant growth is highly sensitive to elevated temperatures. High temperature (HT) triggers the auxin biosynthesis-dependent growth in aerial tissues. On the other hand, the contribution of auxin to HT-induced root growth is currently under debate. Here we show that the putative intracellular auxin carrier PIN-LIKES 6 (PILS6) is a negative regulator of organ growth and that its abundance is highly sensitive to HT  » get the pre-print

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