Stellenausschreibungen


Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: "Erkennungsmechanismen von Metallionenbelastungen bei Pflanzen" 

Cell walls do not only protect the cell but serves also as signaling platform between the extracellular environment and the intracellular physiology. We are interested in the molecular analysis of cell wall associated receptor kinases which are involved in metal ion sensing. By using molecular genetics, CRISPR/Cas9 gene editing and cell biological approaches this project aims to uncover the role of a class of receptor kinases in the model plant Arabidopsis thaliana.

Keywords: cell wall, kinases, signal transduction, cadmium, nickel, zinc, lead, copper

For more detailed information contact Marie-Theres Hauser

Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: "Veränderung der Pektinmethylierung bei Metallionenbelastung"

The pectin component, homogalacturonan (HG), is synthesized and incorporated in the cell wall in a highly methylesterified form. Upon demethylation HG establishes intramolecular calcium/metal ion bridges, the so-called egg-box structures important for cell wall stiffening. This project tackles the role of HGs in heavy metal ion capturing and sensing using the model plant Arabidopsis thaliana and molecular genetics, cell biological and biochemical approaches.   

Keywords: cell wall, pectin, signal transduction, heavy metals, trace elements, cadmium, nickel, zinc, lead, copper

For more detailed information contact Marie-Theres Hauser

Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: "Regulation der Translation bei Stress exponierten Pflanzen"

Major efforts were invested in studying transcriptional regulation upon abiotic stresses while understanding of the regulation of protein synthesis is lagging behind. Comparative genomic and proteomic analyses have shown that mRNA and protein levels do not correlate perfectly, highlighting the important contribution of translational control and protein stability to gene expression. This project employs a novel method in combination with genetics to identify regulators of translation which are important for abiotic stress responses.

Keywords: UV-B, heavy metals, protein synthesis, translation initiation factors  

For more detailed information contact Marie-Theres Hauser

Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: "Epigenetische Regulation ribosomaler RNA"                        

Transcriptome-wide analyses have shown that RNA modifications occur on all major classes of RNA including ribosomal RNA. Recently a member of an evolutionarily conserved family of methyltransferases has been identified in the model plant Arabidopsis thaliana which is responsible to methylate the 25S rRNA of the large ribosomal subunit. With the help of genetic and molecular biological analyses the project analyses the dynamics of rRNA methylation during development and in response to abiotic stressors and aims to reveal the functional role of this epigenetic modification on the translational efficiency, growth and fecundity. 

Keywords: UV-B, heavy metals, protein synthesis, rRNA, RNA methylation, methyltransferases 

For more detailed information contact Marie-Theres Hauser

Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: "Untersuchungen über Metallionen induzierten epigenetischen Veränderungen bei Pflanzen"                 

Plants are sessile organisms and cannot escape adverse environmental conditions as for example the increase of heavy metals and trace elements in soils due to air pollution but also heavy use of fertilizers and fungicides. In this project the impact on the epigenome should be analyzed upon chronic exposure to elevated concentrations of metal ions. Quantitative gene expression analyses will be combined with experiments using epigenetically silenced reporter lines of the model plant Arabidopsis thaliana.      

Keywords: cadmium, nickel, zinc, lead, copper, DNA methylation, chromatin remodeling, small RNA expression, gene silencing

For more detailed information contact Marie-Theres Hauser

Pflanzengenetik (Arbeitsgruppe Marie-Theres Hauser)

Master Thesis: “Molecular characterization of a cell wall associated kinase involved in heavy metal sensing” 

Phytoremediation, the extraction of heavy metals and other contaminates through plants might be a cost effective method to restore metal polluted soils. We have isolated a heavy metal induced cell wall associated kinase of a heavy metal tolerant willow. To characterize its function we transferred this gene fused with a fluorescing protein gene to the model plant Arabidopsis thaliana. To characterize the protein domains necessary for the cell wall association and triggering heavy metal responses, microscopic analyses (CLSM) will be employed combined with cell fractionations and Western blots. Since it is anticipated that the project needs many hours on high end confocal laser scanning microscopes only students should take this project which love microscopy. 

Keywords: Microscopy, Western blot, heavy metals

For more detailed information contact Marie-Theres Hauser

Abteilung für Mikrobielle Genetik und Pathogen-Interaktionen
Fungal Genomics Unit (Joseph Strauss)

PhD Thesis  detaillierte Information

Abteilung für Mikrobielle Genetik und Pathogen-Interaktionen
Fungal Genomics Unit (Joseph Strauss)

   ⇒ Master Thesis   detaillierte Information

Abteilung für Mikrobielle Genetik und Pathogen-Interaktionen
Fungal Genomics Unit (Joseph Strauss)

   ⇒ Master Thesis   detaillierte Information